DOI: https://doi.org/10.22377/ijgp.v3i2.76
S. Arokiyaraj, K. Perinbam, P. Agastian1, R. Mohan Kumar2
Department of PG and Research, Sathyabama University, Chennai – 119, 1Department of Plant Biology and Biotechnology, Loyola College, Chennai – 34, 2Astagiri Herbal Research Foundations, Chennai, India
Abstract
The leaves of Vitex agnus-castus was sequentially extracted in hexane, ethyl acetate, methanol and aqueous medium and studied for in vitro antibacterial property. The ethyl acetate extract was found to be most active against all the bacterial species tested except K. pneumoniae. The best MIC value (0.312 mg/ml) was seen against MRSA. Active ethyl acetate extract was further studied for HPTLC fingerprint and phytochemical analysis. HPTLC analysis confirmed segregation of eight individual compounds with individual Rf
values and peak area percentage. The results of phytochemical screening of extract revealed the presence of terpenoids, steroids, flavonoids and carbohydrates. This analysis revealed the high antibacterial activity in active ethyl acetate from Vitex agnus- castus.
Keywords: Antibacterial activity, drug resistant, minimum inhibitory concentration, phytochemical analysis, vitex agnus-castus